Schuurs T A, Schaeffer E A, Wessels J G
Department of Plant Biology, Groningen Biomolecular Sciences and Technology Institute, University of Groningen, Haren, The Netherlands.
Genetics. 1997 Oct;147(2):589-96. doi: 10.1093/genetics/147.2.589.
After introduction of extra copies of the SC3 hydrophobin gene into a wild-type strain of Schizophyllum commune, gene silencing was observed acting on both endogenous and introduced SC3 genes in primary vegetative transformants. Nuclear run-on experiments indicated that silencing acted at the transcriptional level. Southern analysis revealed that cytosine methylation of genomic DNA occurred. Moreover, SC3 silencing was suppressed by exposure to 5-azacytidine during growth. After growth of SC3-suppressed colonies from homogenized mycelium or from colonies stored at 4 degrees, SC3 transcription was restored. However, after prolonged growth SC3 silencing was again observed. Introduction of a promoterless SC3 fragment into wild type gave less SC3 silencing.
将额外的SC3疏水蛋白基因拷贝导入裂褶菌野生型菌株后，在初级营养转化体中观察到基因沉默作用于内源性和导入的SC3基因。核转录实验表明沉默作用于转录水平。Southern分析显示基因组DNA发生了胞嘧啶甲基化。此外，在生长过程中暴露于5-氮杂胞苷可抑制SC3沉默。从匀浆菌丝体或4℃保存的菌落中生长出SC3抑制菌落后，SC3转录得以恢复。然而，长时间生长后再次观察到SC
