Myers S L, Brandt K D, O'Connor B, Widmer W R, Albrecht M
Indiana University School of Medicine, and Indiana University Multipurpose Arthritis and Musculoskeletal Diseases Center, Indianapolis, USA.
Arthritis Rheum. 1997 Oct;40(10):1756-9. doi: 10.1002/art.1780401005.
To characterize, for the first time, periosteal new bone formation in a well-established canine model of accelerated osteoarthritis (OA) with features of neuropathic arthropathy.
Seven dogs underwent left L4-S1 dorsal root ganglionectomy (DRG), followed 3 weeks later by transection of the anterior cruciate ligament of the ipsilateral knee (ACLT). Eight weeks thereafter, a postmortem examination was performed to assess the severity of cartilage changes of OA and the formation of new bone on the distal femur and proximal tibia in the cruciate-deficient limb.
As described previously, extensive full-thickness ulceration of the articular cartilage was present in the unstable knee of every dog. The femoral shaft immediately proximal to the condyles in the unstable limb was consistently wider (mean +/- SD diameter 22.4 +/- 2.2 mm) than that in the contralateral limb (19.9 +/- 1.3 mm; P = 0.01). Xeroradiography and histologic examination of the distal femur revealed extensive formation of woven bone on the periosteal surfaces of the medial, lateral, and anterior aspects of the femoral shaft in the OA limb of every dog. These bony changes were not seen in radiographs of dogs that underwent DRG with the cruciate ligament left intact (n = 8) or of neurologically intact dogs that underwent ACLT (n = 7) and were examined 24 weeks after surgery.
Formation of new periosteal bone on the distal femur and tibia is a feature of this model of accelerated OA that is not seen in the conventional ACLT model of OA in the neurologically intact dog. This observation suggests that interruption of sensory input from the limb may affect the regulation of osteogenesis in the mechanically unstable joint.
首次对具有神经性关节病特征的成熟犬类加速性骨关节炎（OA）模型中的骨膜新骨形成进行特征描述。
七只犬接受左L4 - S1背根神经节切除术（DRG），3周后对同侧膝关节前交叉韧带进行横断（ACLT）。此后八周，进行尸检以评估OA软骨变化的严重程度以及交叉韧带缺失肢体中股骨远端和胫骨近端新骨的形成情况。
如先前所述，每只犬不稳定膝关节均存在广泛的全层关节软骨溃疡。不稳定肢体中紧邻髁的股骨干始终比对侧肢体宽（平均±标准差直径22.4±2.2毫米），对侧为19.9±1.3毫米；P = 0.01。对股骨远端的干板X线照相和组织学检查显示，每只犬OA肢体股骨干内侧、外侧和前侧骨膜表面均有广泛的编织骨形成。在交叉韧带完整接受DRG的犬（n = 8）或接受ACLT的神经功能正常犬（n = 7）术后24周进行检查的X线片中未见到这些骨质变化。
股骨远端和胫骨新骨膜骨的形成是该加速性OA模型的一个特征，在神经功能正常犬的传统ACLT OA模型中未见此现象。这一观察结果表明，来自肢体的感觉输入中断可能会影响机械不稳定关节中骨生成的调节。
