酿酒酵母核苷酸切除修复缺陷突变体中DNA损伤后RNA聚合酶II合成的恢复
Recovery of RNA polymerase II synthesis following DNA damage in mutants of Saccharomyces cerevisiae defective in nucleotide excision repair.
作者信息
Reagan M S, Friedberg E C
机构信息
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA.
出版信息
Nucleic Acids Res. 1997 Nov 1;25(21):4257-63. doi: 10.1093/nar/25.21.4257.
Abstract
We have measured the kinetics of the recovery of mRNA synthesis in the inducible GAL10 and RNR3 genes after exposure of yeast cells to ultraviolet (UV) radiation. Such recovery is abolished in mutant strains defective in nucleotide excision repair (NER) of DNA, including a rad23 mutant. Mutants defective in the RAD7 or RAD16 genes, which are required for the repair of the non-transcribed strand but not the transcribed strand of transcriptionally active genes, show slightly faster recovery of RNA synthesis than wild-type strains. A strain deleted of the RAD26 gene, which is known to be required for strand-specific NER in yeast, manifested delayed recovery of mRNA synthesis, whereas a rad28 mutant, which does not show defective strand-specific repair, showed normal kinetics of recovery. Measurement of the recovery of expression of selected individual yeast genes by Northern analysis following exposure of cells to UV radiation apparently correlates directly with the capacity of cells for strand-specific NER.
摘要
我们已经测定了酵母细胞暴露于紫外线（UV）辐射后，可诱导的GAL10和RNR3基因中mRNA合成恢复的动力学。在DNA核苷酸切除修复（NER）存在缺陷的突变菌株中，包括rad23突变体，这种恢复被消除。RAD7或RAD16基因存在缺陷的突变体，这些基因是转录活性基因非转录链而非转录链修复所必需的，其RNA合成恢复速度比野生型菌株略快。缺失RAD26基因的菌株，已知该基因是酵母中链特异性NER所必需的，其mRNA合成恢复延迟，而不表现出链特异性修复缺陷的rad28突变体，其恢复动力学正常。通过Northern分析测定细胞暴露于UV辐射后选定的单个酵母基因表达的恢复情况，显然与细胞进行链特异性NER的能力直接相关。
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本文引用的文献
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Human transcription-repair coupling factor CSB/ERCC6 is a DNA-stimulated ATPase but is not a helicase and does not disrupt the ternary transcription complex of stalled RNA polymerase II.人类转录修复偶联因子CSB/ERCC6是一种DNA刺激的ATP酶，但不是解旋酶，也不会破坏停滞的RNA聚合酶II的三元转录复合物。
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