Roche M E, Oda R P, Landers J P
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA.
Biotechnol Prog. 1997 Sep-Oct;13(5):659-68. doi: 10.1021/bp970079k.
Capillary electrophoresis (CE) is a versatile micro/macroanalytical technique gaining widespread usage for the separation and analysis of ionic substances. It has captured the attention of those working in a variety of arenas focused on biologically-active molecules. Its appealing characteristics include unprecedented mass sensitivity and the ability for precise, automated electrophoretic separation of microvolume samples with relatively short analysis times. Such versatility in bioanalysis makes it an inviting replacement for some of the labor-intensive, time-consuming methodologies performed via electrophoretic gels. Moreover, CE compliments the ease and speed of HPLC while eliminating the problem of excessive solvent volume usage and hazardous waste disposal. Further attractive characteristics of this technology include the analyses of a diverse spectrum of analytes, ranging from small organic ions to macromolecular protein complexes and DNA. While combining some of the most robust aspects of traditional electrophoresis, chromatography, and capillary technology, recent CE research and development has focused on avenues leading to improving detection and understanding and employing the basic chemistry of CE vis-a-vis new applications.
毛细管电泳（CE）是一种用途广泛的微/宏观分析技术，在离子物质的分离和分析中得到广泛应用。它已引起了从事各种关注生物活性分子领域工作的人们的注意。其吸引人的特性包括前所未有的质量灵敏度，以及能够以相对较短的分析时间对微量样品进行精确、自动的电泳分离。这种在生物分析中的多功能性使其成为一些通过电泳凝胶进行的劳动密集型、耗时方法的诱人替代品。此外，CE兼具高效液相色谱（HPLC）的简便性和速度，同时消除了溶剂用量过多和危险废物处理的问题。该技术的其他吸引人的特性包括对从有机小离子到大分子蛋白质复合物和DNA等各种分析物的分析。在结合了传统电泳、色谱和毛细管技术的一些最强大方面的同时，最近的毛细管电泳研发工作集中在改进检测、理解以及针对新应用运用毛细管电泳基本化学原理的途径上。
