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[氧化葡萄糖酸杆菌中的棉子糖代谢]

[Raffinose metabolism in Gluconobacter oxydans].

作者信息

Loĭtsianskaia M S, Uspenskaia S N

出版信息

Mikrobiologiia. 1976 Mar-Apr;45(2):229-33.

PMID:933868
Abstract

Metabolism of raffinose has been examined in experiments with the growing culture and washed cells of Gluconobacter oxydans L-1. Degradtion of the trisaccharide was found to be catalyzed by levansucrase, levan being synthesized, and melibiose and small quantitites of fructose being liberated in the reaction. Melibiose is not hydrolyzed and is not used by the bacterium as a source of carbon, but is oxidized to melibionic acid. Fructose is assimilated by the bacterium in constructive metabolism, being oxidized to gluconic, 2-ketogluconic acids and 5-ketofructose.

摘要

已在氧化葡萄糖杆菌L-1的生长培养物和洗涤过的细胞实验中研究了棉子糖的代谢。发现三糖的降解由蔗糖酶催化,合成了果聚糖,反应中释放出蜜二糖和少量果糖。蜜二糖不被水解,也不被细菌用作碳源,而是被氧化为蜜二糖酸。果糖在建设性代谢中被细菌同化,被氧化为葡萄糖酸、2-酮葡萄糖酸和5-酮果糖。

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