甘蓝型油菜花粉壁油质蛋白的特性分析
Characterization of oleosins in the pollen coat of Brassica oleracea.
作者信息
Ruiter R K, Van Eldik G J, Van Herpen R M, Schrauwen J A, Wullems G J
机构信息
Department of Experimental Botany, University of Nijmegen, The Netherlands.
出版信息
Plant Cell. 1997 Sep;9(9):1621-31. doi: 10.1105/tpc.9.9.1621.
Abstract
Mature Brassica oleracea pollen grains are covered with a lipophilic pollen coat containing a variety of proteins. Screening of an anther cDNA expression library for the coding sequences of such proteins resulted in the isolation of a number of cDNA clones encoding glycine-rich oleosins. The proteins were shown to be attached to the lipophilic coat material only and to be absent elsewhere in the plant. Within the coat, several forms of the pollen coat oleosin with different molecular weights were detected. The forms are encoded by different transcripts that originate from a single gene. Expression of this gene is restricted to the tapetum and is quantitatively regulated by the water content of the anther. Similar oleosins were found in the pollen coat of B. alboglobra and B. napus.
摘要
成熟的甘蓝花粉粒覆盖着一层含有多种蛋白质的亲脂性花粉壁。通过筛选花药cDNA表达文库以寻找此类蛋白质的编码序列，从而分离出了许多编码富含甘氨酸油质蛋白的cDNA克隆。结果表明，这些蛋白质仅附着于亲脂性壁物质上，在植物的其他部位并不存在。在花粉壁内，检测到了几种分子量不同的花粉壁油质蛋白形式。这些形式由源自单个基因的不同转录本编码。该基因的表达仅限于绒毡层，并受花药含水量的定量调控。在白甘蓝和油菜的花粉壁中也发现了类似的油质蛋白。
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