Shimotohno K W, Miwa I, Endo T
Kyoritsu College of Pharmacy, Tokyo, Japan.
Biosci Biotechnol Biochem. 1997 Sep;61(9):1459-64. doi: 10.1271/bbb.61.1459.
The gene from Bacillus brevis TT02-8 encoding arginase was cloned into Escherichia coli, and its nucleotide sequence was identified. The nucleotide sequence contained an open reading frame that encoded a polypeptide of 298 amino acid residues with a predicted molecular weight of 31,891, which was consistent with that previously calculated for arginase purified from this bacterium. Comparison of the deduced amino acid sequence of the B. brevis TT02-8 arginase with that of the prokaryotic and eukaryotic arginases of Bacillus caldovelox, Bacillus subtilis, Agrobacterium Ti plasmid C58, Saccharomyces cerevisiae, Coccidioides immitis, Xenopus laevis, Rana catesbeiana, rat liver, and human liver, showed 33-66% of the sequences to be similar; there were several highly conserved regions. Arginase activity was detected in Escherichia coli cells transformed with an expression plasmid of the cloned arginase gene.
将来自短短芽孢杆菌TT02 - 8的编码精氨酸酶的基因克隆到大肠杆菌中，并鉴定其核苷酸序列。该核苷酸序列包含一个开放阅读框，其编码一个由298个氨基酸残基组成的多肽，预测分子量为31,891，这与先前从该细菌中纯化的精氨酸酶的计算分子量一致。将短短芽孢杆菌TT02 - 8精氨酸酶的推导氨基酸序列与嗜热栖热芽孢杆菌、枯草芽孢杆菌、根癌土壤杆菌Ti质粒C58、酿酒酵母、粗球孢子菌、非洲爪蟾、牛蛙、大鼠肝脏和人肝脏的原核和真核精氨酸酶的氨基酸序列进行比较，结果显示33 - 66%的序列相似；存在几个高度保守的区域。在用克隆的精氨酸酶基因的表达质粒转化的大肠杆菌细胞中检测到了精氨酸酶活性。
