枯草芽孢杆菌（纳豆芽孢杆菌）NR-1γ-谷氨酰转肽酶基因ggt的DNA序列

DNA sequence of Bacillus subtilis (natto) NR-1 gamma-glutamyltranspeptidase gene, ggt.

作者信息

Ogawa Y, Sugiura D, Motai H, Yuasa K, Tahara Y

机构信息

Research and Development Division, Kikkoman Corporation, Chiba, Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Sep;61(9):1596-600. doi: 10.1271/bbb.61.1596.

DOI:10.1271/bbb.61.1596

PMID:9339568

Abstract

The ggt encoding gamma-glutamyltranspeptidase (GGT) from Bacillus subtilis (natto) was cloned and sequenced. The DNA sequence contains a single open reading frame of 1761 bp that might be translated to a protein of 587 amino acid residues, and indicates that B. subtilis (natto) GGT is synthesized as prepro-GGT and processed later into large and small subunits. The putative catabolite responsive element (CRE) was located upstream of the ggt coding region.

摘要

对来自枯草芽孢杆菌（纳豆芽孢杆菌）的编码γ-谷氨酰转肽酶（GGT）的ggt进行了克隆和测序。该DNA序列包含一个1761 bp的单一开放阅读框，可能被翻译为一个由587个氨基酸残基组成的蛋白质，这表明枯草芽孢杆菌（纳豆芽孢杆菌）GGT是以前原GGT的形式合成的，随后加工成大亚基和小亚基。推测的分解代谢物反应元件（CRE）位于ggt编码区的上游。