枯草芽孢杆菌中γ-谷氨酰转肽酶编码基因的鉴定、序列分析及表达
Identification, sequence, and expression of the gene encoding gamma-glutamyltranspeptidase in Bacillus subtilis.
作者信息
Xu K, Strauch M A
机构信息
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.
出版信息
J Bacteriol. 1996 Jul;178(14):4319-22. doi: 10.1128/jb.178.14.4319-4322.1996.
Abstract
The Bacillus subtilis gene encoding gamma-glutamyltranspeptidase (GGT) activity encodes a protein of 587 amino acids having extensive homologies with other procaryotic GGTs. Inactivation of the gene abolished all measurable GGT activity, which in the wild type was found mainly to be excreted into the medium commencing at the end of vegetative growth.
摘要
编码γ-谷氨酰转肽酶（GGT）活性的枯草芽孢杆菌基因编码一种由587个氨基酸组成的蛋白质，该蛋白质与其他原核生物GGT具有广泛的同源性。该基因的失活消除了所有可测量的GGT活性，在野生型中，这种活性主要在营养生长末期开始分泌到培养基中。
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