地衣芽孢杆菌和枯草芽孢杆菌中谷氨酸对聚谷氨酸合成的调控
Regulation of polyglutamic acid synthesis by glutamate in Bacillus licheniformis and Bacillus subtilis.
作者信息
Kambourova M, Tangney M, Priest F G
机构信息
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
出版信息
Appl Environ Microbiol. 2001 Feb;67(2):1004-7. doi: 10.1128/AEM.67.2.1004-1007.2001.
Abstract
The synthesis of polyglutamic acid (PGA) was repressed by exogenous glutamate in strains of Bacillus licheniformis but not in strains of Bacillus subtilis, indicating a clear difference in the regulation of synthesis of capsular slime in these two species. Although extracellular gamma-glutamyltranspeptidase (GGT) activity was always present in PGA-producing cultures of B. licheniformis under various growth conditions, there was no correlation between the quantity of PGA and enzyme activity. Moreover, the synthesis of PGA in the absence of detectable GGT activity in B. subtilis S317 indicated that this enzyme was not involved in PGA biosynthesis in this bacterium. Glutamate repression of PGA biosynthesis may offer a simple means of preventing unwanted slime production in industrial fermentations using B. licheniformis.
摘要
在地衣芽孢杆菌菌株中，外源谷氨酸会抑制聚谷氨酸（PGA）的合成，但在枯草芽孢杆菌菌株中则不会，这表明这两个物种在荚膜黏液合成调控方面存在明显差异。尽管在各种生长条件下地衣芽孢杆菌产生PGA的培养物中始终存在细胞外γ-谷氨酰转肽酶（GGT）活性，但PGA的量与酶活性之间没有相关性。此外，在枯草芽孢杆菌S317中未检测到GGT活性时PGA的合成表明该酶不参与该细菌中PGA的生物合成。谷氨酸对PGA生物合成的抑制作用可能为防止在地衣芽孢杆菌的工业发酵中产生不必要的黏液提供一种简单方法。
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