McCormick R M, Nelson R J, Alonso-Amigo M G, Benvegnu D J, Hooper H H
Soane BioSciences, Hayward, California 94545-3716, USA.
Anal Chem. 1997 Jul 15;69(14):2626-30. doi: 10.1021/ac9701997.
Microfabricated electrophoretic separation devices have been produced by an injection-molding process. The strategy for producing the devices involved solution-phase etching of a master template on a silicon wafer, followed by electroforming more durable injection-molding masters in nickel from the silicon master. One of the nickel electroforms was then used to prepare an injection mold insert, from which microchannel chips in an acrylic substrate were mass-produced. The microchannel devices were used to demonstrate high-resolution separations of double-stranded DNA fragments with total run times of less than 3 min. Run-to-run and chip-to-chip reproducibility was good, with relative standard deviation values below 1% for the run-to-run data and in the range of 2-3% for the chip-to-chip comparisons. Such devices could lead to the production of low-cost, single-use electrophoretic chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, random primary library screening, and rapid immunoassay testing.
微制造电泳分离装置已通过注塑工艺生产出来。制造这些装置的策略包括对硅片上的主模板进行溶液相蚀刻，然后从硅主模板上电铸出更耐用的镍注塑主模板。然后使用其中一个镍电铸模板制备注塑模具镶件，由此批量生产出丙烯酸基板中的微通道芯片。这些微通道装置用于演示双链DNA片段的高分辨率分离，总运行时间不到3分钟。批间和芯片间的重现性良好，批间数据的相对标准偏差值低于1%，芯片间比较的相对标准偏差值在2%-3%范围内。此类装置可能会带来适合多种分离应用的低成本一次性电泳芯片的生产，包括DNA大小测定、DNA测序、随机初级文库筛选和快速免疫测定测试。
