Okazaki M, Sasamoto K, Muramatsu T, Hosaki S
Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Chiba, Japan.
Clin Chem. 1997 Oct;43(10):1885-90.
HDL-cholesterol (HDL-C) values measured by precipitation (sodium phosphotungstate-MgCl2) and direct methods were compared with those obtained by HPLC with a new column (TSKgel Lipopropak) and an eluent (TSKeluent LP-1). The HDL-C values determined by the precipitation method were significantly (P <0.001) lower than those by the HPLC method, whereas the HDL-C values by the direct method were slightly but significantly higher (P <0.02) than those by the HPLC method. A quantitative HPLC analysis of the cholesterol concentration in HDL and non-HDL fractions in the supernatant of serum separated by precipitation reagents with different MgCl2 concentrations ranging from 7.3 to 44 mmol/L revealed that reagents with >22 mmol/L MgCl2 precipitated part of HDL as well as non-HDL lipoproteins. The HPLC method providing quantitative and qualitative information with high precision was regarded as being a reliable approach for HDL-C assay. The HPLC can be also used to evaluate alternative methods for cholesterol assay.
通过沉淀法（磷钨酸钠 - 氯化镁）和直接法测定的高密度脂蛋白胆固醇（HDL-C）值，与使用新色谱柱（TSKgel Lipopropak）和洗脱液（TSKeluent LP-1）的高效液相色谱法（HPLC）所获得的值进行了比较。通过沉淀法测定的HDL-C值显著低于（P <0.001）HPLC法测定的值，而直接法测定的HDL-C值略高于但显著高于（P <0.02）HPLC法测定的值。对用不同氯化镁浓度（范围为7.3至44 mmol/L）的沉淀试剂分离的血清上清液中HDL和非HDL组分中的胆固醇浓度进行定量HPLC分析，结果显示氯化镁浓度>22 mmol/L的试剂会沉淀部分HDL以及非HDL脂蛋白。提供高精度定量和定性信息的HPLC方法被认为是一种可靠的HDL-C测定方法。HPLC还可用于评估胆固醇测定的替代方法。
