Kobayashi K, Shimada A, Yamano Y, Umemura T
Department of Veterinary Pathology, Tottori University, Japan.
J Vet Med Sci. 1997 Sep;59(9):819-23. doi: 10.1292/jvms.59.819.
A canine metallothionein cDNA obtained from the liver of a cadmium-treated beagle was cloned and sequenced. Asn at position 4 conserved among all mammalian metallothionein-1 and metallothionein-2 is replaced by Asp in the canine metallothionein cDNA clone. Because the acidic amino acid doesn't exist at either position 10 or 11 in the deduced amino acid sequence, it is supposed that this cDNA is derived from canine metallothionein-1 mRNA. Northern blot analysis using the cDNA as a probe revealed the induction of the canine metallothionein mRNA expression in the liver and kidney of a cadmium-treated beagle. Thus, the canine metallothionein cDNA obtained in the present study should provide an useful tool for the molecular investigation of metallothionein in dog.
从经镉处理的比格犬肝脏中获得的犬金属硫蛋白cDNA被克隆并测序。在所有哺乳动物金属硫蛋白-1和金属硫蛋白-2中第4位保守的天冬酰胺在犬金属硫蛋白cDNA克隆中被天冬氨酸取代。由于在推导的氨基酸序列中第10位和第11位均不存在酸性氨基酸，推测该cDNA来源于犬金属硫蛋白-1 mRNA。用该cDNA作为探针进行的Northern印迹分析显示，经镉处理的比格犬肝脏和肾脏中犬金属硫蛋白mRNA表达被诱导。因此，本研究中获得的犬金属硫蛋白cDNA应为犬金属硫蛋白的分子研究提供一个有用的工具。
