通过逆转录聚合酶链反应（RT-PCR）从犬、猫、牛、马和兔肝脏信使核糖核酸（mRNA）中对抑制素互补脱氧核糖核酸（cDNA）进行部分克隆。

Partial cloning of prohibitin cDNA from canine, feline, bovine, equine, and rabbit liver mRNA by RT-PCR.

作者信息

Matsuyama S, Kubo K, Ohashi F, Takamori Y

机构信息

Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.

出版信息

J Vet Med Sci. 1997 Mar;59(3):201-3. doi: 10.1292/jvms.59.201.

DOI:10.1292/jvms.59.201

PMID:9101479

Abstract

Prohibitin is the protein which has an inhibitory function in cell growth, and its gene is suggested to be one of putative tumor suppressor genes. In this report, we described a partial cloning of prohibitin cDNAs from canine, feline, bovine, equine, and rabbit liver mRNAs by RT-PCR, and their homology analysis. The sequences of these RT-PCR products were compared with each other as well as those reported for human and rat. The homology in this region of prohibitin cDNA was approximately 90%, and the amino acid sequence of each RT-PCR product shared more than 95% identity. Therefore, it is concluded that all the RT-PCR products are a part of prohibitin cDNA of each animal.

摘要

抑制素是一种在细胞生长中具有抑制功能的蛋白质，其基因被认为是假定的肿瘤抑制基因之一。在本报告中，我们描述了通过逆转录聚合酶链反应（RT-PCR）从犬、猫、牛、马和兔肝脏mRNA中部分克隆抑制素cDNA及其同源性分析。将这些RT-PCR产物的序列相互比较，以及与已报道的人和大鼠的序列进行比较。抑制素cDNA该区域的同源性约为90%，每个RT-PCR产物的氨基酸序列具有超过95%的同一性。因此，可以得出结论，所有的RT-PCR产物都是每种动物抑制素cDNA的一部分。