Matsuyama S, Kubo K, Ohashi F, Takamori Y
Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.
J Vet Med Sci. 1997 Mar;59(3):201-3. doi: 10.1292/jvms.59.201.
Prohibitin is the protein which has an inhibitory function in cell growth, and its gene is suggested to be one of putative tumor suppressor genes. In this report, we described a partial cloning of prohibitin cDNAs from canine, feline, bovine, equine, and rabbit liver mRNAs by RT-PCR, and their homology analysis. The sequences of these RT-PCR products were compared with each other as well as those reported for human and rat. The homology in this region of prohibitin cDNA was approximately 90%, and the amino acid sequence of each RT-PCR product shared more than 95% identity. Therefore, it is concluded that all the RT-PCR products are a part of prohibitin cDNA of each animal.
抑制素是一种在细胞生长中具有抑制功能的蛋白质，其基因被认为是假定的肿瘤抑制基因之一。在本报告中，我们描述了通过逆转录聚合酶链反应（RT-PCR）从犬、猫、牛、马和兔肝脏mRNA中部分克隆抑制素cDNA及其同源性分析。将这些RT-PCR产物的序列相互比较，以及与已报道的人和大鼠的序列进行比较。抑制素cDNA该区域的同源性约为90%，每个RT-PCR产物的氨基酸序列具有超过95%的同一性。因此，可以得出结论，所有的RT-PCR产物都是每种动物抑制素cDNA的一部分。
