Osimani Andrea, Garofalo Cristiana, Clementi Francesca, Tavoletti Stefano, Aquilanti Lucia
Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, via Brecce Bianche, Ancona 60131, Italy.
Int J Environ Res Public Health. 2014 Oct 17;11(10):10824-37. doi: 10.3390/ijerph111010824.
ATP bioluminescence monitoring and traditional microbiological analyses (viable counting of total mesophilic aerobes, coliforms and Escherichia coli) were used to evaluate the effectiveness of Sanitation Standard Operating Procedures (SSOP) at a university canteen which uses a HACCP-based approach. To that end, 10 cleaning control points (CPs), including food contact surfaces at risk of contamination from product residues or microbial growth, were analysed during an 8-month monitoring period. Arbitrary acceptability limits were set for both microbial loads and ATP bioluminescence readings. A highly significant correlation (r = 0.99) between the means of ATP bioluminescence readings and the viable counts of total mesophilic aerobes was seen, thus revealing a strong association of these parameters with the level of surface contamination. Among CPs, the raw meat and multi-purpose chopping boards showed the highest criticalities. Although ATP bioluminescence technology cannot substitute traditional microbiological analyses for the determination of microbial load on food contact surfaces, it has proved to be a powerful tool for the real time monitoring of surface cleanliness at mass catering plants, for verify the correct application of SSOP, and hence for their implementation/revision in the case of poor hygiene.
采用ATP生物发光监测和传统微生物分析方法（对总嗜温需氧菌、大肠菌群和大肠杆菌进行活菌计数），以评估一所采用基于危害分析与关键控制点（HACCP）方法的大学食堂卫生标准操作程序（SSOP）的有效性。为此，在为期8个月的监测期内，对10个清洁控制点（CPs）进行了分析，这些控制点包括有受产品残留或微生物生长污染风险的食品接触表面。为微生物负荷和ATP生物发光读数设定了任意可接受限度。ATP生物发光读数平均值与总嗜温需氧菌活菌计数之间存在高度显著的相关性（r = 0.99），从而揭示了这些参数与表面污染水平之间的紧密关联。在各清洁控制点中，生肉和多功能砧板的关键性最高。尽管ATP生物发光技术不能替代传统微生物分析来测定食品接触表面的微生物负荷，但它已被证明是一种用于大规模餐饮场所表面清洁实时监测、验证SSOP正确应用的有力工具，因此在卫生状况不佳时可用于其实施/修订。
