Ayté J, Leis J F, DeCaprio J A
Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA.
Mol Cell Biol. 1997 Nov;17(11):6246-54. doi: 10.1128/MCB.17.11.6246.
Depending on environmental conditions, Schizosaccharomyces pombe can remain in the stationary phase or enter into either premitotic or premeiotic DNA synthesis. This decision point is known as Start. In the mitotic cell cycle, regulation of G1/S-specific gene expression is dependent upon the MBF (Mlu1 binding factor) complex, known to contain p85cdc10 and p72res1. Here we demonstrate that p73res2 controls cell cycle progression via its participation in the MBF complex, interacting directly with both p85cdc10 and p72res1. In contrast, when cells enter into meiosis, the MBF complex is disrupted, and p73res2 shifts its regulatory function towards the transactivation of genes required for meiotic progression. These observations suggest that p73res2 plays a pivotal role at Start and constitutes an example of a transcription factor involved in the control of both mitotic and meiotic progression.
根据环境条件，粟酒裂殖酵母可以停留在静止期，或者进入有丝分裂前或减数分裂前的DNA合成阶段。这个决策点被称为“起始点（Start）”。在有丝分裂细胞周期中，G1/S特异性基因表达的调控依赖于MBF（Mlu1结合因子）复合物，已知该复合物包含p85cdc10和p72res1。在这里，我们证明p73res2通过参与MBF复合物来控制细胞周期进程，它直接与p85cdc10和p72res1相互作用。相比之下，当细胞进入减数分裂时，MBF复合物被破坏，p73res2将其调节功能转向减数分裂进程所需基因的反式激活。这些观察结果表明，p73res2在起始点发挥关键作用，并构成了一个参与有丝分裂和减数分裂进程控制的转录因子的例子。
