Ghosh P, O'Dell M, Narasimhan P T, Fraser S E, Jacobs R E
Beckman Institute, Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Neuroimage. 1994 Nov;1(4):345-9. doi: 10.1006/nimg.1994.1019.
We present a three-dimensional (3D) digital atlas of a mouse lemur head at 60-micron cubic voxel isotropic resolution. It was constructed from a 3D proton magnetic resonance image (MRI) acquired at 500 MHz. It shows views of 3D volume-rendering and movies of contiguous 2D plane cuts in three orthogonal directions. This MR data set was acquired using a spin-echo pulse sequence under conditions of strong T2 and significant diffusion weighting. Experimental parameters were optimized to provide strong intrinsic contrast between gray and white matter. Familiar anatomical structures are clearly identifiable. Fine fiber tracts, laminations of cortices, details of the inner ears, and layering in the lateral geniculate nuclei are all visible. Anatomical identifications of structures in representative slices selected from the atlas are presented. We also describe some difficulties and trade-offs encountered in microscopic resolution MRI. We note that this type of atlas does not suffer from the spatial distortions and slice registration problems introduced by standard histological techniques. Future in vivo longitudinal studies will provide atlases describing in detail the development of the primate brain.
我们展示了一个小鼠狐猴头部的三维（3D）数字图谱，其体素分辨率为各向同性的60微米立方。它由在500兆赫获取的三维质子磁共振图像（MRI）构建而成。它展示了三维体绘制视图以及在三个正交方向上连续二维平面切割的动态影像。该磁共振数据集是在强T2和显著扩散加权条件下使用自旋回波脉冲序列获取的。实验参数经过优化，以在灰质和白质之间提供强烈的固有对比度。熟悉的解剖结构清晰可辨。细纤维束、皮质分层、内耳细节以及外侧膝状核的分层都清晰可见。呈现了从图谱中选取的代表性切片中结构的解剖学标识。我们还描述了在微观分辨率MRI中遇到的一些困难和权衡。我们注意到，这种类型的图谱不存在标准组织学技术所引入的空间扭曲和切片配准问题。未来的体内纵向研究将提供详细描述灵长类大脑发育的图谱。
