Mikic Z D, Brankov M Z, Tubic M V, Lazetic A B
Clinic of Orthopaedic Surgery and Traumatology, Faculty of Medicine, Novi Sad, Yugoslavia.
Arthroscopy. 1997 Oct;13(5):579-83. doi: 10.1016/s0749-8063(97)90183-3.
Transplantation of 25 fresh-frozen medial menisci was studied in 15 adult dogs. Before implantation, the allografts were deep-frozen and stored at -70 degrees C for 6 weeks to 18 months. The animals were killed 2 to 8 months postoperatively, and their knees and transplants were examined macroscopically and histologically. Complete healing of the allografts was found in 19 knees, incomplete in 3, and healing by massive fibrovascular tissue in 3 knees. Some shrinkage of the transplants taken 2 to 4 months after the surgery was observed; however, the 6- and 8-month specimens appeared grossly normal. Histologically, all transplants displayed a characteristic decrease in the number of cells, but this was significantly less pronounced in the 6- and 8-month specimens. Some degenerative alterations were found in all transplanted knees, but was obviously less pronounced in areas covered by the allografts and in the 6- and 8-month specimens. It is concluded that the transplantation of the fresh-frozen menisci could be successful; although the transplants are subjected to a remodeling process, they appear to function normally and protect the articular cartilage. The technique of conservation by freezing at -70 degrees C offers the advantage of an effective meniscal banking.
对15只成年犬进行了25个新鲜冷冻内侧半月板移植的研究。植入前，将同种异体移植物深度冷冻并在-70℃下储存6周至18个月。术后2至8个月处死动物，对其膝关节和移植物进行宏观和组织学检查。19个膝关节的同种异体移植物完全愈合，3个不完全愈合，3个膝关节通过大量纤维血管组织愈合。观察到术后2至4个月取出的移植物有一些萎缩；然而，6个月和8个月的标本大体上看起来正常。组织学上，所有移植物的细胞数量均有特征性减少，但在6个月和8个月的标本中这种减少明显不那么明显。在所有移植的膝关节中均发现了一些退行性改变，但在同种异体移植物覆盖的区域以及6个月和8个月的标本中明显不那么明显。结论是新鲜冷冻半月板移植可能成功；尽管移植物会经历重塑过程，但它们似乎功能正常并能保护关节软骨。在-70℃下冷冻保存技术具有有效储存半月板的优势。
