Fukuyama H, Ouchi Y, Matsuzaki S, Ogawa M, Yamauchi H, Nagahama Y, Kimura J, Yonekura Y, Shibasaki H, Tsukada H
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Neuroimage. 1996 Jun;3(3 Pt 1):195-201. doi: 10.1006/nimg.1996.0021.
We evaluated the cholinergic mechanism underlying focal cortical vascular response to neuronal activation, using positron emission tomography for use on animals to measure cerebral blood flow and glucose metabolism activation upon vibrotactile stimulation in cats. Bromopyruvate, which blocks acetylcholine synthesis through inhibition of the production of acetyl CoA, was injected into the cerebral cortex and basal forebrain as well as the sphenopalatine ganglion, all of which have been confirmed to supply cholinergic terminals to the cerebral cortex. Although glucose metabolism was preserved, indicating that the neuronal activities were enhanced, cerebral blood flow increase during cortical neuronal activation was abolished by bromopyruvate injection into only the cerebral cortex and not other cholinergic systems. We conclude that the cholinergic intrinsic neurons control the focal cerebral blood flow increase in response to neuronal activation.
我们使用正电子发射断层扫描技术评估了局灶性皮质血管对神经元激活的胆碱能机制，该技术用于测量猫在触觉刺激下的脑血流量和葡萄糖代谢激活情况。将通过抑制乙酰辅酶A的产生来阻断乙酰胆碱合成的溴丙酮酸注入大脑皮层、基底前脑以及蝶腭神经节，所有这些部位均已被证实向大脑皮层提供胆碱能终末。尽管葡萄糖代谢得以保留，表明神经元活动增强，但仅向大脑皮层而非其他胆碱能系统注射溴丙酮酸后，皮质神经元激活期间的脑血流量增加被消除。我们得出结论，胆碱能内在神经元控制着对局灶性脑血流量增加的神经元激活反应。
