Kay L E, Gardner K H
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Curr Opin Struct Biol. 1997 Oct;7(5):722-31. doi: 10.1016/s0959-440x(97)80084-x.
Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR experiments and new deuterium-labeling strategies have significantly increased the scope of structural problems that can now be addressed by solution NMR methods. To date, a number of structures of proteins of 30 kDa have been solved using multidimensional 15N,13C,2H NMR techniques, and this molecular weight limit will probably be surpassed in the near future.
核磁共振（NMR）仪器的改进、更高的磁场强度、新颖的NMR实验以及新的氘标记策略，显著扩大了目前可用溶液NMR方法解决的结构问题范围。迄今为止，已经使用多维15N、13C、2H NMR技术解析了许多30 kDa蛋白质的结构，并且在不久的将来这个分子量限制可能会被突破。
