罗德岛红鸡和白来航鸡近交系杂交中的随机扩增多态性DNA标记

Random amplified polymorphic DNA markers in crosses between inbred lines of Rhode Island red and White Leghorn chickens.

作者信息

Wei R, Dentine M R, Bitgood J J

机构信息

Poultry Science Department, University of Wisconsin, Madison 53706, USA.

出版信息

Anim Genet. 1997 Aug;28(4):291-4. doi: 10.1111/j.1365-2052.1997.00130.x.

DOI:10.1111/j.1365-2052.1997.00130.x

PMID:9345725

Abstract

Reciprocal crosses and backcrosses were conducted between inbred Rhode Island Red and White Leghorn chickens differentiated for egg production and egg quality traits. Random amplified polymorphic DNA (RAPD) markers distinguishing inbred lines were detected. Twenty-two polymorphic bands were found from screening 120 single 10-mer random primers of which two were consistent with sex-linked markers. Of 90 pairwise two-point linkage analyses completed for the autosomal markers, four close linkages (8.2 cM to 14.9 cM) were significantly different from zero.

摘要

在产蛋量和蛋品质性状上存在差异的近交罗德岛红鸡和白来航鸡之间进行了正反交和回交。检测到区分近交系的随机扩增多态性DNA（RAPD）标记。从120个单10聚体随机引物的筛选中发现了22个多态性条带，其中两个与性连锁标记一致。在对常染色体标记完成的90次两点连锁分析中，四个紧密连锁（8.2厘摩至14.9厘摩）与零显著不同。