Levin I, Santangelo L, Cheng H, Crittenden L B, Dodgson J B
Department of Microbiology, Michigan State University, East Lansing 48824.
J Hered. 1994 Mar-Apr;85(2):79-85. doi: 10.1093/oxfordjournals.jhered.a111427.
We have developed an autosomal genetic linkage map of the chicken genome using a subpanel of 52 DNAs from a previously described reference backcross mapping population. The population derived from a cross of an inbred Red Jungle Fowl male and a highly inbred White Leghorn female. The backcross subpanel used was made up of offspring of a single F1 male with four White Leghorn females. Ninety-eight markers consisting of classical and erythrocyte antigen genes, restriction fragment length polymorphisms, random amplified polymorphic DNA, and chicken CR1 repeat-element polymorphisms were typed. Seventy-two of these markers were resolved into 19 linkage groups. Four of the linkage groups were assigned to chromosomes 1, 4, and 17. Four linkage groups were associated with linkage groups published earlier. Linkages within approximately 27 cM can be detected with a lod score of 3 with the panel used. The preliminary map contains approximately 590 cM within the linkage groups, and approximately 70% of the randomly selected markers fell in one of the groups; however, a considerable portion of the genome may remain outside of the existing linkage groups. These markers greatly expand the existing linkage map of the chicken genome.
我们利用一个由52个DNA组成的亚组，从先前描述的参考回交作图群体中构建了鸡基因组的常染色体遗传连锁图谱。该群体源自一只近交原鸡雄性与一只高度近交白来航雌性的杂交。所使用的回交亚组由一只F1雄性与四只白来航雌性的后代组成。对包括经典和红细胞抗原基因、限制性片段长度多态性、随机扩增多态性DNA以及鸡CR1重复元件多态性在内的98个标记进行了分型。其中72个标记被解析为19个连锁群。四个连锁群被定位到1号、4号和17号染色体上。四个连锁群与先前发表的连锁群相关。使用该亚组，在约27厘摩范围内的连锁关系可通过3的lod分数检测到。初步图谱在连锁群内包含约590厘摩，约70%的随机选择标记落在其中一个群体中；然而，基因组的相当一部分可能仍处于现有连锁群之外。这些标记极大地扩展了现有的鸡基因组连锁图谱。
