Mikuni J, Morohoshi N
Laboratory of Wood Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
FEMS Microbiol Lett. 1997 Oct 1;155(1):79-84. doi: 10.1111/j.1574-6968.1997.tb12689.x.
A second laccase gene, CVLG1, was isolated from Coriolus versicolor. CVLGI encodes a precursor protein of 526 amino acids which contains a 23-amino acid signal sequence, and the coding region is interrupted by 11 introns. The number of potential N-glycosylation sites in this product is 12 and the greatest among that of polyporales laccases. Moreover, this protein shares about 70% homology with other polyporales laccases. Genomic Southern analysis showed that C. versicolor laccases are encoded by more than four genes including CVLG1 and a transposed allele of this gene.
从云芝中分离出了第二个漆酶基因CVLG1。CVLGI编码一个由526个氨基酸组成的前体蛋白，该蛋白包含一个23个氨基酸的信号序列，其编码区被11个内含子打断。该产物中潜在的N-糖基化位点数量为12个，在多孔菌漆酶中是最多的。此外，该蛋白与其他多孔菌漆酶具有约70%的同源性。基因组Southern分析表明，云芝漆酶由包括CVLG1及其该基因的一个转座等位基因在内的四个以上基因编码。
