Tantivanich S, Klongkamnuankarn K, Desakorn V
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1997 Jun;15(2):99-103.
DOT ELISA was compared with RT-PCR and tissue culture to detect RSV from nasopharyngeal aspirates. DOT ELISA had diagnostic sensitivity and specificity of 65.62% and 93.92%, respectively. The results indicate that DOT ELISA can be used for screening detection of RSV from clinical specimens and is suitable for small laboratories in the provincial areas of developing countries.
采用斑点酶联免疫吸附试验（DOT ELISA）与逆转录聚合酶链反应（RT-PCR）及组织培养法对鼻咽抽吸物中的呼吸道合胞病毒（RSV）进行检测比较。DOT ELISA的诊断敏感性和特异性分别为65.62%和93.92%。结果表明，DOT ELISA可用于临床标本中RSV的筛查检测，适用于发展中国家省级地区的小型实验室。
