Klongpanichpak S, Temcharoen P, Toskulkao C, Apibal S, Glinsukon T
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
J Med Assoc Thai. 1997 Sep;80 Suppl 1:S121-8.
Stevioside, a sweet-tasting diterpene glycoside derived from Stevia rebaudiana, and steviol, a product from enzymatic hydrolysis of stevioside, were tested for mutagenic activity by the in vitro Ames test, a preincubation method, using Salmonella typhimurium TA 98 and TA 100 as the tester strains, either in the presence or absence of metabolic activating system derived from the sodium phenobarbital and 5,6-benzoflavone pretreated liver S9 fractions from various animal species including rat, mouse, hamster and guinea pig. Stevioside and steviol at the concentrations up to 50 mg and 2 mg per plate, respectively showed no mutagenic effect on both tester strains either in the presence or absence of metabolic activating system. However, at the high concentration both stevioside and steviol showed some toxic effects on both tester strains. The toxic effect was decreased in the presence of the metabolic activating system.
甜菊糖苷是一种从甜叶菊中提取的具有甜味的二萜糖苷，而甜菊醇是甜菊糖苷经酶水解后的产物。通过体外艾姆斯试验（一种预孵育方法），以鼠伤寒沙门氏菌TA 98和TA 100作为测试菌株，在有或没有由苯巴比妥钠和5,6 - 苯并黄酮预处理的来自包括大鼠、小鼠、仓鼠和豚鼠等各种动物物种的肝脏S9组分衍生的代谢激活系统的情况下，对甜菊糖苷和甜菊醇的致突变活性进行了测试。甜菊糖苷和甜菊醇在每平板分别高达50毫克和2毫克的浓度下，无论有无代谢激活系统，对两种测试菌株均未显示出致突变作用。然而，在高浓度下，甜菊糖苷和甜菊醇对两种测试菌株均显示出一些毒性作用。在有代谢激活系统的情况下，毒性作用有所降低。
