Krogstad A L, Lönnroth P, Larson G, Wallin B G
Department of Dermatology, Sahlgren University Hospital, Göteborg, Sweden.
J Invest Dermatol. 1997 Nov;109(5):632-5. doi: 10.1111/1523-1747.ep12337620.
The psoriatic plaque contains an increased number of mast cells that are thought to have an important role in the initiation and maintenance of psoriatic lesions through the release of mediators such as histamine, proteoglycans, lipid mediators, and cytokines. It is not known, however, whether the interstitial concentration of histamine (and other mediators) is truly increased in the psoriatic plaque. The aim of the present study was to examine histamine concentration and histamine release from involved and uninvolved skin of psoriatic patients. Intracutaneous microdialysis was performed in lesional and nonlesional skin of 23 psoriatic subjects. The relative recovery of histamine was assessed after calibration in situ to approximately 76% in both lesional and nonlesional skin. The interstitial histamine concentration was 32 +/- 3 nmol per liter in lesional skin and 13 +/- 1 nmol per liter in nonlesional skin (mean +/- SEM) (p < 0.001). Dermal histamine release was estimated according to the Fick principle after measurements of the arterialized venous plasma histamine concentration (3 +/- 1 nmol per liter) and blood flow and was found to be 10-fold increased in lesional compared with nonlesional skin. The results are compatible with the hypothesis that mast cells in lesional skin secrete an increased amount of histamine that may contribute to the immunostimulation and inflammation in the psoriatic plaque.
银屑病斑块中肥大细胞数量增加，这些肥大细胞被认为通过释放组胺、蛋白聚糖、脂质介质和细胞因子等介质，在银屑病皮损的起始和维持中发挥重要作用。然而，尚不清楚银屑病斑块中组胺（和其他介质）的间质浓度是否真的增加。本研究的目的是检测银屑病患者受累皮肤和未受累皮肤中的组胺浓度及组胺释放情况。对23例银屑病患者的皮损和非皮损皮肤进行了皮内微透析。原位校准后，皮损和非皮损皮肤中组胺的相对回收率均约为76%。皮损皮肤中间质组胺浓度为每升32±3纳摩尔，非皮损皮肤中为每升13±1纳摩尔（平均值±标准误）（p<0.001）。在测量动脉化静脉血浆组胺浓度（每升3±1纳摩尔）和血流量后，根据菲克原理估算真皮组胺释放量，发现皮损皮肤中的释放量比非皮损皮肤增加了10倍。这些结果与以下假设相符：皮损皮肤中的肥大细胞分泌的组胺量增加，这可能有助于银屑病斑块中的免疫刺激和炎症反应。
