Combates N J, Chuong C M, Stenn K S, Prouty S M
Skin Biology Technology Resource Center, Johnson and Johnson CPWW, Skillman, New Jersey 08558, USA.
J Invest Dermatol. 1997 Nov;109(5):672-8. doi: 10.1111/1523-1747.ep12337725.
The hair cycle involves remodeling of cells and of cell groups into a complex follicular structure. During skin appendage development, adhesion molecules such as neural cell adhesion molecule (NCAM) and deleted in colon carcinoma (DC) participate in the formation of cell groups. NCAM has been found to be expressed in the mesenchyme during mouse hair follicle induction. DCC expression has been observed in the epithelial cells of the developing feather. We postulate that these two molecules may also define cell groups in the cycling hair follicle. Here we report their spatio-temporal expression patterns during the depilation-induced murine hair cycle. NCAM expression was also examined in positive and negative hair-inductive follicular papilla cell lines. Throughout the hair cycle, DCC expression was confined to the basal keratinocytes of the epidermis and the epithelial portion of the hair follicle. During mid-anagen, two types of deleted in colon carcinoma staining were observed. One was a cell surface pattern seen in the epithelial cells in the bulge region where the follicular stem cells reside. The other was a diffuse cytoplasmic staining pattern in the transient hair follicle epithelia located below the bulge region. Prominent NCAM staining was observed in the follicular papilla throughout the hair cycle and was accompanied by weak staining of the matrix epithelia. NCAM expression correlated with hair induction by a follicular papilla cell line. The results suggest that DCC and NCAM define the permanent cell groups of the hair follicle and that NCAM is important for hair induction.
毛发周期涉及细胞及细胞群重塑为复杂的毛囊结构。在皮肤附属器发育过程中，诸如神经细胞黏附分子（NCAM）和结肠癌缺失基因（DCC）等黏附分子参与细胞群的形成。已发现NCAM在小鼠毛囊诱导期的间充质中表达。在发育中的羽毛上皮细胞中观察到了DCC的表达。我们推测这两种分子也可能在周期性毛囊中界定细胞群。在此我们报告它们在拔毛诱导的小鼠毛发周期中的时空表达模式。还在阳性和阴性毛发诱导毛囊乳头细胞系中检测了NCAM的表达。在整个毛发周期中，DCC的表达局限于表皮的基底角质形成细胞和毛囊的上皮部分。在生长期中期，观察到了两种结肠癌缺失基因染色类型。一种是在毛囊干细胞所在的隆突区上皮细胞中看到的细胞表面模式。另一种是在隆突区下方的短暂毛囊上皮中的弥漫性胞质染色模式。在整个毛发周期中，在毛囊乳头中均观察到明显的NCAM染色，并伴有基质上皮的弱阳性染色。NCAM表达与毛囊乳头细胞系诱导毛发相关。结果表明，DCC和NCAM界定了毛囊的永久性细胞群，且NCAM对毛发诱导很重要。
