Müller-Röver S, Peters E J, Botchkarev V A, Panteleyev A, Paus R
Department of Dermatology, Charité, Humboldt-Universität zu Berlin, Berlin, Germany.
J Histochem Cytochem. 1998 Dec;46(12):1401-10. doi: 10.1177/002215549804601209.
Hair follicle development, growth (anagen), and regression (catagen) largely result from bidirectional epithelial-mesenchymal interactions whose molecular basis is still unclear. Because adhesion molecules are critically involved in pattern formation and because the fundamental importance of neural cell adhesion molecule (NCAM) for feather development has been demonstrated, we studied the protein expression patterns of NCAM during hair follicle development and regression in the C57BL/6 mouse model. During murine hair follicle development, NCAM immunoreactivity (IR) was first detected on epithelial hair placodes and later on selected keratinocytes in the distal outer root sheath. Mesenchymal NCAM immunoreactivity (IR) was noted on fibroblasts of the future dermal papilla (DP) and the perifollicular connective tissue sheath. Fetal hair follicle elongation coincided with strong, ubiquitous dermal NCAM IR, which remained strong until the follicles entered into their first neonatal catagen. At this time, the strong interfollicular dermal NCAM IR decreased substantially. During consecutive hair cycles, mesenchymal NCAM IR was seen exclusively on DP and perifollicular connective tissue sheath fibroblasts and on the trailing cells of regressing catagen hair follicles. These highly restricted and developmentally controlled expression patterns suggest an important role for NCAM in hair follicle topobiology during morphogenesis and cyclic remodeling of this miniorgan.
毛囊的发育、生长（生长期）和退化（退行期）很大程度上源于上皮-间充质的双向相互作用，其分子基础仍不清楚。由于黏附分子在模式形成中起关键作用，且神经细胞黏附分子（NCAM）对羽毛发育的重要性已得到证实，我们研究了C57BL/6小鼠模型中毛囊发育和退化过程中NCAM的蛋白表达模式。在小鼠毛囊发育过程中，NCAM免疫反应性（IR）首先在上皮毛基板上被检测到，随后在远端外根鞘的特定角质形成细胞上被检测到。在未来的真皮乳头（DP）和成毛囊结缔组织鞘的成纤维细胞上观察到间充质NCAM免疫反应性（IR）。胎儿毛囊的伸长与强烈、普遍存在的真皮NCAM IR同时出现，这种情况一直持续到毛囊进入第一个新生儿退行期。此时，强烈的毛囊间真皮NCAM IR大幅下降。在连续的毛发周期中，间充质NCAM IR仅在DP和成毛囊结缔组织鞘成纤维细胞以及退行期毛囊的尾随细胞上可见。这些高度受限且受发育调控的表达模式表明，NCAM在这个小器官的形态发生和周期性重塑过程中的毛囊拓扑生物学中起重要作用。
