Shin H W, Shinotsuka C, Torii S, Murakami K, Nakayama K
Institute of Biological Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki.
J Biochem. 1997 Sep;122(3):525-30. doi: 10.1093/oxfordjournals.jbchem.a021784.
The dynamin family of GTP-binding proteins are implicated in vesicular transport. These include mammalian dynamins I, II, III, and yeast Vps1p and Dnm1p. Dynamin is involved in the formation of clathrin-coated vesicles at the plasma membrane. On the other hand, Vps1p and Dnm1p appear to be involved in transport from the late Golgi compartment to vacuoles and in an endocytic process, respectively. In this study, we identified a novel human protein, named Dnm1p/Vps1p-like protein (DVLP). It resembled more closely Dnm1p and Vps1p than dynamins not only in the primary structure but also in the domain organization. DVLP mRNA was expressed ubiquitously, suggesting that this protein plays a fundamental role in cellular function. Immunofluorescence analysis of cells expressing epitope-tagged DVLP revealed that it showed a diffused perinuclear staining pattern that was not superimposed on that of the marker protein for the Golgi apparatus, trans-Golgi network, lysosomes, endosomes, or endoplasmic reticulum. These data suggest that DVLP is not involved in the formation of known coated vesicles.
GTP结合蛋白的发动蛋白家族与囊泡运输有关。这些包括哺乳动物的发动蛋白I、II、III，以及酵母的Vps1p和Dnm1p。发动蛋白参与质膜上网格蛋白包被囊泡的形成。另一方面，Vps1p和Dnm1p似乎分别参与从高尔基体晚期区室到液泡的运输以及一个内吞过程。在本研究中，我们鉴定出一种新的人类蛋白，命名为Dnm1p/Vps1p样蛋白（DVLP）。它不仅在一级结构上，而且在结构域组织上，比发动蛋白更类似于Dnm1p和Vps1p。DVLP mRNA在全身广泛表达，表明该蛋白在细胞功能中起基本作用。对表达表位标记的DVLP的细胞进行免疫荧光分析显示，它呈现出一种弥漫性的核周染色模式，该模式与高尔基体、反式高尔基体网络、溶酶体、内体或内质网的标记蛋白的染色模式不重叠。这些数据表明DVLP不参与已知包被囊泡的形成。
