Damms T, Ross J R, Duplessie M D, Klintworth G K
Medizinische Hochschule, Hanover, Germany.
Graefes Arch Clin Exp Ophthalmol. 1997 Oct;235(10):662-6. doi: 10.1007/BF00946944.
We characterized the neovascularization that follows the intracorneal injection of bovine albumin (BA) in rabbits as a model of corneal angiogenesis.
New Zealand white rabbits received intracorneal injections of phosphate-buffered saline with and without various amounts of BA. The rabbits were co-sensitized or pre-sensitized by intramuscular BA or were not sensitized. The corneal vascular response was quantified by ranking photographs taken periodically after the injection.
In pre-sensitized animals, blood vessels were apparent within 4 days and reached maximum intensity 14 days after the intracorneal injection. Corneas also vascularized in non-sensitized rabbits, but a larger dose (> 0.2 mg BA) was required than in pre-sensitized animals (> 0.02 mg BA). Vascularization began later in non-sensitized animals and was less extensive than in pre-sensitized animals.
The intracorneal injection of BA is a reproducible model of corneal angiogenesis in rabbits and should allow the involved immunological mechanisms to be elucidated.
我们将兔角膜内注射牛白蛋白（BA）后出现的新生血管形成作为角膜血管生成的一种模型进行了特征描述。
新西兰白兔接受角膜内注射含不同剂量BA或不含BA的磷酸盐缓冲盐水。通过肌肉注射BA使兔子产生共同致敏或预先致敏，或不进行致敏。注射后定期拍摄照片，通过对照片进行分级来量化角膜血管反应。
在预先致敏的动物中，角膜内注射后4天血管明显可见，并在14天达到最大强度。未致敏的兔子角膜也会发生血管化，但所需剂量（>0.2mg BA）比预先致敏的动物（>0.02mg BA）更大。未致敏动物的血管化开始时间较晚，且程度不如预先致敏的动物。
角膜内注射BA是兔角膜血管生成的一种可重复模型，应有助于阐明其中涉及的免疫机制。
