Claros-Chacaltana Flor Diana Yokoay, Aldrovani Marcela, Kobashigawa Karina Kamachi, Padua Ivan Ricardo Martinez, Valdetaro Gisele Pereira, de Barros Sobrinho Alexandre Augusto Franchi, Abreu Thaís Guimarães Morato, Laus José Luiz
Ophthalmology Unit, Department of Veterinary Clinics and Surgery, School of Agricultural and Veterinarian Sciences (FCAV), São Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP, CEP 14884-900, Brazil.
Int Ophthalmol. 2019 May;39(5):1123-1135. doi: 10.1007/s10792-018-0922-2. Epub 2018 Apr 26.
To evaluate the effect of metronidazole ophthalmic solutions on corneal neovascularization (CNV) in a rat model.
A chemical burn was created in the right central cornea of 40 rats. Animals were randomized and distributed into four study groups (n = 10 rats per group) designated Met_0.1%, Met_0.5%, sham, and untreated groups. Chemical-burned corneas in the Met_0.1% and Met_0.5% groups received ophthalmic solutions of 0.1 and 0.5% metronidazole, respectively. Corneas in the sham group received phosphate-buffered saline (metronidazole diluent). All treated eyes received ophthalmic solution at intervals of 6 h, for up to 30 days. Untreated corneas received no treatment. CNV was evaluated postinjury using corneal photographs at different evaluation time points. The main CNV outcome measures were: burn intensity, index of CNV, and percentage of vascularized corneal area. Five rats from each group were euthanized, on days 15 and 30; the samples were collected for histological analyses. Differences with P < 0.05 were considered significant.
CNV was observed in the eyes from day 7 postinjury. However, the indices of CNV for the Met_0.1% and Met_0.5% groups were smaller than those for the sham and untreated groups (P < 0.05). Furthermore, corneas treated with 0.1 or 0.5% metronidazole had smaller vascularized areas compared to control corneas. On histological study, the presence of blood vessels confirmed clinical outcomes.
Regular instillation of 0.1 or 0.5% metronidazole had a significant inhibitory effect for CNV on chemical burns induced in a rat model.
评估甲硝唑滴眼液对大鼠模型角膜新生血管化（CNV）的影响。
在40只大鼠的右眼中央角膜制造化学伤。将动物随机分为四个研究组（每组n = 10只大鼠），分别为0.1%甲硝唑组、0.5%甲硝唑组、假手术组和未治疗组。0.1%甲硝唑组和0.5%甲硝唑组的化学伤角膜分别接受0.1%和0.5%的甲硝唑滴眼液。假手术组的角膜接受磷酸盐缓冲盐水（甲硝唑稀释剂）。所有治疗的眼睛每隔6小时接受一次滴眼液，持续30天。未治疗的角膜不接受任何治疗。在不同评估时间点使用角膜照片评估损伤后的CNV。主要的CNV结局指标为：烧伤强度、CNV指数和角膜血管化面积百分比。每组5只大鼠在第15天和第30天实施安乐死；收集样本进行组织学分析。P < 0.05的差异被认为具有统计学意义。
在损伤后第7天观察到眼睛出现CNV。然而，0.1%甲硝唑组和0.5%甲硝唑组的CNV指数小于假手术组和未治疗组（P < 0.05）。此外，与对照角膜相比，用0.1%或0.5%甲硝唑治疗的角膜血管化面积更小。组织学研究显示，血管的存在证实了临床结果。
定期滴注0.1%或0.5%甲硝唑对大鼠模型化学伤诱导的CNV具有显著抑制作用。
