Jensen K T, Carstens J, Ivarsen P, Pedersen E B
Research Laboratory of Nephrology and Hypertension, Aarhus University Hospital, Denmark.
Scand J Clin Lab Invest. 1997 Oct;57(6):529-40. doi: 10.3109/00365519709084604.
A new, fast and reliable radioimmunoassay for measurement of brain natriuretic peptide (BNP) in human plasma has been developed and its application is reported in healthy subjects and in patients with congestive heart failure, chronic renal failure, liver cirrhosis and essential hypertension. The antibody was raised in rabbits, the tracer was made by the iodogen method and polyethylene glycol was used for separation of free and bound tracer. BNP was extracted from plasma using Sep-Pak C18 cartridges. The recovery of unlabelled BNP added to plasma was 77.5 +/- 6.2% (mean +/- SD). The detection limit in plasma was 0.55 pmol l-1. No cross-reactivity existed with the natriuretic peptides ANP, CNP or urodilatin. In 124 healthy subjects the mean BNP was 1.8 +/- 1.0 pmol l-1 (SD), range 0.6-5.5. BNP increased slightly with age, was higher in women than men and had no circadian rhythm. In eight patients with congestive heart failure the median BNP level was 30.5 pmol l-1, range 3.9-65.3. In 14 patients with chronic renal failure the median BNP level was 50.5 pmol l-1, range 10.9-219.8 before dialysis, and 38.0 pmol l-1, range 9.4-180.0 immediately following dialysis. In 25 patients with liver cirrhosis the median BNP value was 7.8 pmol l-1, range 1.2-43.1. There was no difference between patients with or without ascites. In 18 medically treated patients with essential hypertension the median BNP level was 5.0 pmol l-1, range 1.2-45.5 pmol l-1.
已开发出一种用于测定人血浆中脑钠肽（BNP）的新型快速可靠放射免疫分析法，并报道了其在健康受试者以及充血性心力衰竭、慢性肾衰竭、肝硬化和原发性高血压患者中的应用情况。该抗体由兔产生，示踪剂采用碘代法制备，聚乙二醇用于分离游离和结合的示踪剂。使用Sep-Pak C18柱从血浆中提取BNP。添加到血浆中的未标记BNP回收率为77.5±6.2%（平均值±标准差）。血浆中的检测限为0.55 pmol l-1。与利钠肽心房钠尿肽（ANP）、C型钠尿肽（CNP）或尿舒张素无交叉反应。在124名健康受试者中，平均BNP为1.8±1.0 pmol l-1（标准差），范围为0.6 - 5.5。BNP随年龄略有升高，女性高于男性，且无昼夜节律。8名充血性心力衰竭患者的BNP中位数水平为30.5 pmol l-1，范围为3.9 - 65.3。14名慢性肾衰竭患者透析前BNP中位数水平为50.5 pmol l-1，范围为10.9 - 219.8，透析后立即为38.0 pmol l-1，范围为9.4 - 180.0。25名肝硬化患者的BNP中位数为7.8 pmol l-1，范围为1.2 - 43.1。有腹水和无腹水的患者之间无差异。18名接受药物治疗的原发性高血压患者的BNP中位数水平为5.0 pmol l-1，范围为1.2 - 45.5 pmol l-1。
