Goldenberger D, Künzli A, Vogt P, Zbinden R, Altwegg M
Department of Medical Microbiology, University of Zürich, Switzerland.
J Clin Microbiol. 1997 Nov;35(11):2733-9. doi: 10.1128/jcm.35.11.2733-2739.1997.
Broad-range PCR amplification of part of the 16S rRNA gene followed by single-strand sequencing was applied to samples of 18 resected heart valves from patients with infective endocarditis. The PCR results were compared with those of cultures of valves and with those of previous blood cultures. For two patients there was agreement with the cultures of the valves; for nine patients there was agreement with the previous blood cultures, which were positive, while the cultures of the valves were negative; a Streptococcus sp. and Tropheryma whippelii each were found in one patient with negative cultures (valve and blood); for two patients the cultures of the valves as well as the PCR results were negative but the blood cultures were positive; for one patient amplification was inhibited; and for two patients the PCR results were positive but the amplicons could not be sequenced. It is concluded that broad-range PCR is a promising tool for patients with culture-negative endocarditis and allows the detection of rare, noncultivable organisms.
对18例感染性心内膜炎患者切除的心脏瓣膜样本进行了16S rRNA基因部分片段的广谱聚合酶链反应（PCR）扩增，随后进行单链测序。将PCR结果与瓣膜培养结果以及既往血培养结果进行比较。有2例患者的结果与瓣膜培养结果一致；9例患者的结果与既往阳性血培养结果一致，而瓣膜培养结果为阴性；在1例培养结果（瓣膜和血液）均为阴性的患者中分别发现了1株链球菌属细菌和1株惠普尔嗜组织细胞菌；有2例患者的瓣膜培养结果及PCR结果均为阴性，但血培养结果为阳性；有1例患者的扩增受到抑制；有2例患者的PCR结果为阳性，但扩增产物无法测序。得出的结论是，广谱PCR对于培养阴性的心内膜炎患者是一种有前景的工具，能够检测出罕见的、不可培养的微生物。
