Ziegler M, Shultz B, Gottschling D, Keilacker N, Michaelis D
Probl Endokrinol (Mosk). 1976 May-Jun;22(3):51-4.
The authors describe characteristics and clinical application of the "ROTOP-insulin-test'' set. Radioimmunological insulin determination is carried out by the principle of reverse titration: 50 ml of plasma is incubated for 18 hours with antiserum. The remaining free valencies of insulin antibodies are bound with the excess of 125I-insulin. Bound 125I-insulin is precipitated after the addition of alcohol. The variation coefficient is 7.4% in one batch and 9.1% in analysis in 48 hours (insulin concentration = 2.08 ng/ml = 49 mcU/ml). The precision index of the standard curve was 0.022. Insulin secretion was analyzed in 107 healthy persons with a normal body weight under conditions of prolonged glucose infusion. Two phases of insulin secretion were shown.
作者描述了“旋转胰岛素试验”套件的特点和临床应用。放射免疫法测定胰岛素是通过反向滴定原理进行的：将50毫升血浆与抗血清孵育18小时。胰岛素抗体的剩余游离价与过量的125I胰岛素结合。加入酒精后，结合的125I胰岛素沉淀。一批次的变异系数为7.4%，48小时分析时为9.1%（胰岛素浓度=2.08纳克/毫升=49微单位/毫升）。标准曲线的精密度指数为0.022。在长时间输注葡萄糖的条件下，对107名体重正常的健康人进行了胰岛素分泌分析。显示出胰岛素分泌的两个阶段。
