Kreutzweiser D P, Ebling P M, Holmes S B
Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste. Marie, Ontario, Canada.
Ecotoxicol Environ Saf. 1997 Oct;38(1):63-70. doi: 10.1006/eesa.1997.1562.
Rainbow trout fingerlings were fed dried krill injected with gypsy moth or spruce budworm nuclear polyhedrosis virus (LdNPV and CfNPV, respectively) at a total dose of 1.4 x 10(7) occlusion bodies (OBs) per fish. By the end of the 21-day experimental period there were no adverse effects on fish survival or behavior and no significant differences in feeding rates or growth between treated and control fish. The internal organs of all fish were examined at the end of the experiment and there were no signs of lesions, discoloration, swelling, hemorrhaging, or other aberrations. Visceral tissues were analyzed with a horseradish peroxidase-labeled whole genomic DNA probe (enhanced chemiluminescence procedure) to detect infection by the NPVs. There were no indications of NPV infection (no positive signals) in stomach and intestinal tract tissues of treated fish. High background signals were obtained from liver samples, but further analyses indicated that these were not due to the presence of LdNPV or CfNPV. The protocols outlined here should be applicable to determining infectivity and effects of genetically modified insect viruses on fish.
给虹鳟鱼苗投喂注射了舞毒蛾核型多角体病毒或云杉芽卷蛾核型多角体病毒（分别为LdNPV和CfNPV）的干磷虾，每尾鱼的总剂量为1.4×10⁷个包涵体（OBs）。到21天实验期结束时，对鱼的存活或行为没有不利影响，处理组和对照组鱼之间的摄食率或生长没有显著差异。在实验结束时检查了所有鱼的内脏器官，没有病变、变色、肿胀、出血或其他异常迹象。用辣根过氧化物酶标记的全基因组DNA探针（增强化学发光法）分析内脏组织，以检测NPV的感染情况。处理组鱼的胃和肠道组织中没有NPV感染的迹象（没有阳性信号）。肝脏样本获得了高背景信号，但进一步分析表明这些不是由于LdNPV或CfNPV的存在。这里概述的方案应该适用于确定转基因昆虫病毒对鱼的感染性和影响。
