mdoH 的失活导致大肠杆菌中结肠酸荚膜多糖的表达增加。
Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli.
作者信息
Ebel W, Vaughn G J, Peters H K, Trempy J E
机构信息
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
出版信息
J Bacteriol. 1997 Nov;179(21):6858-61. doi: 10.1128/jb.179.21.6858-6861.1997.
Abstract
Capsule gene (cps) expression, which normally occurs at low levels in Escherichia coli lon+ cells, increased 38-fold in lon+ cells carrying a Tn10::delta kan insertion mapping to 24 min on the E. coli chromosome. Null mutations in rcsA, rcsB, or rcsC abolished the effect of the Tn10::delta kan insertion. Sequencing of both sides of the Tn10::delta kan insertion localized the insertion to the previously reported mdoH gene, which encodes a protein involved in biosynthesis of membrane-derived oligosaccharides (MDOs). A model suggesting that the periplasmic levels of MDOs act to signal RcsC to activate cps expression is proposed.
摘要
荚膜基因（cps）的表达在大肠杆菌lon⁺细胞中通常处于低水平，而在携带一个定位在大肠杆菌染色体24分钟处的Tn10::δkan插入片段的lon⁺细胞中，其表达增加了38倍。rcsA、rcsB或rcsC中的无效突变消除了Tn10::δkan插入的影响。对Tn10::δkan插入片段两侧进行测序，将插入定位到先前报道的mdoH基因，该基因编码一种参与膜衍生寡糖（MDOs）生物合成的蛋白质。提出了一个模型，表明MDOs的周质水平起到向RcsC发出信号以激活cps表达的作用。
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