Chen J C, Lee Y
Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan, 20224.
Arch Environ Contam Toxicol. 1997 Oct;33(3):290-7. doi: 10.1007/s002449900256.
Macrobrachium rosenbergii that had been exposed individually for 24 h to 0 (control), 2, 5, 10 mg/L nitrite-N (nitrite as nitrogen) at 4. 3 and 7.7 pH levels were examined for hemolymph nitrite-N, oxyhemocyanin, protein, acid-base balance, ion concentrations, and ammonia-N (ammonia as nitrogen) excretion. Hemolymph oxyhemocyanin, protein, pH, HCO3- , TCO2, osmolality, and ion concentrations were inversely related to ambient nitrite-N concentration and were lower at pH 4.3. However, hemolymph nitrite-N, PO2 and PCO2 levels, and ammonia-N excretion were directly related to ambient nitrite-N, and were higher at pH 4.3. Ambient nitrite-N and pH level interacted to cause changes in hemolymph nitrite-N, oxyhemocyanin, protein, PO2, and pH levels. It is concluded that for M. rosenbergii following nitrite exposure, the incorporated nitrite causes a decrease of pH and an increase of PO2 in the hemolymph where it reduces oxyhemocyanin level; disturbs nitrogen excretion, ion regulation, and respiratory gas exchange; and may lead to a decrease of oxygen-carrying capacity, which are affected more at low pH.
将罗氏沼虾分别暴露于pH值为4.3和7.7的0（对照）、2、5、10 mg/L亚硝酸盐氮（以氮计的亚硝酸盐）环境中24小时后，检测其血淋巴中的亚硝酸盐氮、氧合血蓝蛋白、蛋白质、酸碱平衡、离子浓度以及氨氮（以氮计的氨）排泄情况。血淋巴中的氧合血蓝蛋白、蛋白质、pH值、碳酸氢根、总二氧化碳、渗透压和离子浓度与环境亚硝酸盐氮浓度呈负相关，且在pH值为4.3时更低。然而，血淋巴中的亚硝酸盐氮、氧分压和二氧化碳分压水平以及氨氮排泄与环境亚硝酸盐氮呈正相关，且在pH值为4.3时更高。环境亚硝酸盐氮和pH值相互作用，导致血淋巴中的亚硝酸盐氮、氧合血蓝蛋白、蛋白质、氧分压和pH值水平发生变化。得出结论：对于暴露于亚硝酸盐后的罗氏沼虾，摄入的亚硝酸盐会导致血淋巴pH值降低、氧分压升高，进而降低氧合血蓝蛋白水平；干扰氮排泄、离子调节和呼吸气体交换；并可能导致携氧能力下降，在低pH值条件下影响更为明显。
