Peng Min, Zeng Digang, Zhu Weilin, Chen Xiuli, Yang Chunling, Liu Qingyun, Li Qiangyong, Wang Huanling, Liu Hong, Liang Jingzhen, Lin Yong, Chen Xiaohan, Zhao Yongzhen
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, China.
Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agriculture University, Wuhan, China.
Front Genet. 2020 Sep 24;11:571880. doi: 10.3389/fgene.2020.571880. eCollection 2020.
Nitrite is a major environmental toxin in aquaculture systems that disrupts multiple physiological functions in aquatic animals. Although nitrite tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. In this study, we constructed a high-density genetic map of with 17,242 single nucleotide polymorphism markers spanning 6,828.06 centimorgans (cM), with an average distance of 0.4 cM between adjacent markers on 44 linkage groups (LGs). Using this genetic map, we identified two markers associated with nitrite tolerance. We then sequenced the transcriptomes of the most nitrite-tolerant and nitrite-sensitive individuals from each of four genetically distinct families (LV-I-4). We found 2,002, 1,983, 1,954, and 1,867 differentially expressed genes in families LV-1, LV-2, LV-3, and LV-4, respectively. By integrating QTL and transcriptomics analyses, we identified a candidate gene associated with nitrite tolerance. This gene was annotated as solute carrier family 26 member 6 (). RNA interference (RNAi) analysis demonstrated that was critical for nitrite tolerance in . The present study increases our understanding of the molecular mechanisms underlying nitrite tolerance in shrimp and provides a basis for molecular-marker-assisted shrimp breeding.
亚硝酸盐是水产养殖系统中的一种主要环境毒素，会扰乱水生动物的多种生理功能。尽管虾对亚硝酸盐的耐受性与水产养殖业的成功生产密切相关，但针对这一性状的遗传学研究却很少。在本研究中，我们构建了一个高密度遗传图谱，该图谱包含17242个单核苷酸多态性标记，覆盖6828.06厘摩（cM），在44个连锁群（LG）上相邻标记之间的平均距离为0.4 cM。利用这一遗传图谱，我们鉴定出了两个与亚硝酸盐耐受性相关的标记。然后，我们对来自四个遗传背景不同的家系（LV-I-4）中最耐亚硝酸盐和最敏感的个体进行了转录组测序。我们分别在LV-1、LV-2、LV-3和LV-4家系中发现了2002个、1983个、1954个和1867个差异表达基因。通过整合QTL和转录组学分析，我们鉴定出了一个与亚硝酸盐耐受性相关的候选基因。该基因被注释为溶质载体家族26成员6（）。RNA干扰（RNAi）分析表明，该基因对虾的亚硝酸盐耐受性至关重要。本研究增进了我们对虾亚硝酸盐耐受性分子机制的理解，并为分子标记辅助对虾育种提供了依据。
