Hollenberg C P, Gellissen G
Institut für Mikrobiologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
Curr Opin Biotechnol. 1997 Oct;8(5):554-60. doi: 10.1016/s0958-1669(97)80028-6.
The methylotrophic yeasts Hansenula polymorpha, Pichia pastoris and Candida boidinii have been developed as production systems for recombinant proteins. The favourable and most advantageous characteristics of these species have resulted in an increasing number off biotechnological applications. As a consequence, these species--especially H. polymorpha and P. pastoris--are rapidly becoming the systems of choice for heterologous gene expression in yeast. Recent advances in the development of these yeasts as hosts for the production of heterologous proteins have provided a catalogue of new applications, methods and system components.
多形汉逊酵母、巴斯德毕赤酵母和博伊丁假丝酵母等甲基营养型酵母已被开发为重组蛋白的生产系统。这些物种的有利且最具优势的特性导致了越来越多的生物技术应用。因此，这些物种——尤其是多形汉逊酵母和巴斯德毕赤酵母——正迅速成为酵母中异源基因表达的首选系统。这些酵母作为异源蛋白生产宿主的开发方面的最新进展提供了一系列新的应用、方法和系统组件。
