Du Pasquier L, Blomberg B, Bernard C C
Eur J Immunol. 1979 Nov;9(11):900-6. doi: 10.1002/eji.1830091112.
Larval Xenopus anti-2,4-dinitrophenyl antibodies of the low-molecular weight type can be analyzed by isoelectric focusing (IEF). Within a clone of genetically identical animals, tadpoles make antibodies whose IEF spectrotypes are shared by most of the individuals. Adults of the same clone also make antibodies of identical spectrotype, but the adult pattern can be very different from the larval one, although both responses are heterogeneous. The larval spectrotypes that one cannot see in a primary adult response can be found if the adult has been primed during larval life and boosted after metamorphosis. The heterogeneity of the response is somewhat lower in tadpoles (up to 12 antibody IEF bands) than in adults (up to 20 antibody IEF bands). The change in the repertoire occurs during metamorphosis at the time of the appearance of two major histocompatibility complex antigens. One, a lymphocyte antigen, appears 10-15 days before the end of metamorphosis, the other, present on red cells (and presumably also on lymphocytes), appears 1.5 month after the end of metamorphosis, as determined by immunofluorescence analysis. During the same period, the syngeneic mixed leukocyte reaction switches from a larval anti-adult to an adult anti-larval reaction.
低分子量类型的非洲爪蟾幼虫抗2,4 -二硝基苯基抗体可通过等电聚焦（IEF）进行分析。在基因相同的动物克隆中，蝌蚪产生的抗体，其IEF光谱类型为大多数个体所共有。同一克隆的成年个体也产生相同光谱类型的抗体，但成年个体的模式可能与幼虫的模式非常不同，尽管两种反应都是异质性的。如果成年个体在幼虫期被致敏并在变态后进行加强免疫，那么在初次成年反应中看不到的幼虫光谱类型就可以被发现。蝌蚪反应的异质性（多达12条抗体IEF条带）比成年个体（多达20条抗体IEF条带）略低。抗体库的变化发生在变态期间，此时出现了两种主要的组织相容性复合体抗原。一种是淋巴细胞抗原，在变态结束前10 - 15天出现，另一种存在于红细胞上（大概也存在于淋巴细胞上），在变态结束后1.5个月出现，这是通过免疫荧光分析确定的。在同一时期，同基因混合淋巴细胞反应从幼虫抗成年反应转变为成年抗幼虫反应。
