Ito A, Wen H, Craig P S, Ma L, Nakao M, Horii T, Pang X L, Okamoto M, Itoh M, Osawa Y, Wang X G, Liu Y H
Department of Parasitology, Gifu University School of Medicine.
Jpn J Med Sci Biol. 1997 Feb;50(1):19-26. doi: 10.7883/yoken1952.50.19.
Western blot analysis was carried out in order to evaluate new serodiagnostic markers, Em18 and Em16, for differentiation of alveolar echinococcosis (AE) from cystic echinococcosis (CE) using 36 serum samples from hydatid patients from Xinjiang, China, where AE and CE are both endemic and one double infection case has been reported. All AE cases except one (5/6) who exhibited a calcified lesion and a single case of double infection showed antibody responses against Em18 and Em16. Some of CE patient sera (6/22) showed antibody response against Em16 except one who showed that against Em18. Analyses of IgG subclass responses against Em18 and Em16 were carried out using all serum samples showing antibody responses against Em18 and/or Em16 (seven CE, five AE, and one AE + CE) and additional samples of three CE and 22 AE from Sichuan, China. IgG4 was the most predominant antibody subclass. Em18 and Em16 were recognized by both IgG4 and IgG1 (in most cases) or by either IgG4 or IgG1 (in minor cases) or by IgG3 (in very rare cases). Neither Em18 nor Em16 was recognized by IgG2 antibodies. The usefulness of Em18 and Em16 as potential new markers for serological differentiation of human AE and CE, respectively, is discussed.
为了评估新的血清学诊断标志物Em18和Em16用于区分肺泡型棘球蚴病（AE）和囊型棘球蚴病（CE），采用来自中国新疆包虫病患者的36份血清样本进行了蛋白质免疫印迹分析，新疆是AE和CE的地方病流行区，且已报告1例双重感染病例。除1例表现为钙化病灶的AE病例（5/6）和1例双重感染病例外，所有AE病例均显示出针对Em18和Em16的抗体反应。部分CE患者血清（6/22）显示出针对Em16的抗体反应，1例显示出针对Em18的抗体反应。使用所有显示针对Em18和/或Em16抗体反应的血清样本（7例CE、5例AE和1例AE+CE）以及来自中国四川的另外3例CE和22例AE样本，对针对Em18和Em16的IgG亚类反应进行了分析。IgG4是最主要的抗体亚类。Em18和Em16在大多数情况下被IgG4和IgG1识别，在少数情况下被IgG4或IgG1识别，在极少数情况下被IgG3识别。IgG2抗体未识别Em18和Em16。本文讨论了Em18和Em16分别作为人类AE和CE血清学鉴别潜在新标志物的实用性。
