Ito A, Osawa Y, Nakao M, Horii T, Okamoto M, Itoh M, Yamashita T
Department of Parasitology, Gifu University School of Medicine, Japan.
J Helminthol. 1995 Dec;69(4):369-71. doi: 10.1017/s0022149x0001498x.
The assay system for antibody responses against Em2, the most specific antigen for serodiagnosis of alveolar echinococcosis (AE), has been established by enzyme-linked immunosorbent assay (ELISA) but not by Western blot assay, since Em2 antigen is not protein but carbohydrate in nature. Recently we reported that previously undescribed protein epitopes, designated Em18 and Em16 due to their molecular weights, were good serologic markers for AE by Western blot analysis. It has been shown that Em18 and Em16 are the only two epitopes recognized by commercially available weak positive (cut off) sera for the Em2plus-ELISA.
针对Em2的抗体反应检测系统已通过酶联免疫吸附测定（ELISA）建立，用于泡型棘球蚴病（AE）血清学诊断的最特异抗原Em2，但未通过蛋白质印迹法建立，因为Em2抗原本质上不是蛋白质而是碳水化合物。最近我们报道，以前未描述的蛋白质表位，因其分子量而命名为Em18和Em16，通过蛋白质印迹分析是AE的良好血清学标志物。已经表明，Em18和Em16是Em2plus-ELISA市售弱阳性（临界值）血清识别的仅有的两个表位。
