Baldi P C, Wanke M M, Loza M E, Monachesi N, Fossati C A
Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET/UBA), Buenos Aires, Argentina.
Vet Microbiol. 1997 Sep;57(2-3):273-81. doi: 10.1016/s0378-1135(97)00134-x.
An antigenic capture ELISA was developed to measure serum antibodies against an 18 kDa cytoplasmic protein of Brucella. This assay was used to detect anti-18 kDa reactivity in sera from 30 dogs having confirmed or suspected brucellosis. Antibodies against the 18 kDa protein were found in 26 of them, which were also positive by the slide agglutination test (2ME-RSAT). The overall correlation (positive and negative results) between the ELISA and 2ME-RSAT tests was 93.3%. Additionally, these sera were assayed by an indirect ELISA using a whole extract of cytoplasmic proteins of B. abortus (LPS-free CYT). The results of both ELISAs were coincident in 28 of 30 (93.3%) dogs having confirmed or suspected brucellosis. When a serological follow-up was performed on some dogs having confirmed brucellosis, antibody titers measured by both ELISAs showed a parallel progression. On the other hand, the capture ELISA showed good specificity, since a positive result was obtained only in 2 of 103 sera from healthy dogs. These preliminary results show that the ELISA for detecting serum antibodies against the 18 kDa cytoplasmic protein of Brucella could be useful for the diagnosis of canine brucellosis. This study also shows that the results obtained with this single protein of Brucella are equivalent to those obtained with the whole extract of cytoplasmic proteins.
开发了一种抗原捕获酶联免疫吸附测定法（ELISA）来检测血清中针对布鲁氏菌18 kDa细胞质蛋白的抗体。该检测方法用于检测30只已确诊或疑似患有布鲁氏菌病的犬血清中抗18 kDa蛋白的反应性。其中26只犬检测到针对18 kDa蛋白的抗体，这些犬通过玻片凝集试验（2ME-RSAT）也呈阳性。ELISA检测与2ME-RSAT检测之间的总体相关性（阳性和阴性结果）为93.3%。此外，使用流产布鲁氏菌细胞质蛋白的全提取物（无脂多糖的CYT）通过间接ELISA对这些血清进行检测。在30只已确诊或疑似患有布鲁氏菌病的犬中，两种ELISA检测结果在28只犬（93.3%）中一致。当对一些已确诊患有布鲁氏菌病的犬进行血清学随访时，两种ELISA检测测得的抗体滴度呈平行变化。另一方面，捕获ELISA显示出良好的特异性，因为在103份健康犬血清中只有2份呈阳性结果。这些初步结果表明，用于检测血清中针对布鲁氏菌18 kDa细胞质蛋白抗体的ELISA可能对犬布鲁氏菌病的诊断有用。本研究还表明，用布鲁氏菌的这种单一蛋白获得的结果与用细胞质蛋白全提取物获得的结果相当。
