布鲁氏菌流产亚种胞质蛋白用作酶联免疫吸附测定（ELISA）中的抗原，可能有助于犬布鲁氏菌病的诊断。
Brucella abortus cytoplasmic proteins used as antigens in an ELISA potentially useful for the diagnosis of canine brucellosis.
作者信息
Baldi P C, Wanke M M, Loza M E, Fossati C A
机构信息
Instituto de Estudios de la Inmunidad Humoral (IDEHU-UBA-CONICET), Buenos Aires, Argentina.
出版信息
Vet Microbiol. 1994 Jul;41(1-2):127-34. doi: 10.1016/0378-1135(94)90142-2.
A preparation of Brucella abortus cytoplasmic proteins was depleted of lipopolysaccharide (LPS) by immunoadsorption with a monoclonal antibody (BC68) specific for the O antigen of B. abortus smooth LPS. This extract was used as antigen in an indirect ELISA for measuring antiprotein humoral immune response in dogs suffering from brucellosis and in healthy controls. All of the affected dogs studied showed IgG antiprotein response, while 2% (2 of 103) of the healthy dogs used as controls gave a positive result. All sera found to be positive by ELISA were also positive by the rapid slide agglutination test. These preliminary results show that the ELISA using B. abortus cytoplasmic proteins could be useful for the specific diagnosis of canine brucellosis.
用针对流产布鲁氏菌光滑型脂多糖O抗原的单克隆抗体（BC68）通过免疫吸附法去除流产布鲁氏菌细胞质蛋白制剂中的脂多糖（LPS）。该提取物用作间接ELISA中的抗原，用于检测患布鲁氏菌病的犬和健康对照犬的抗蛋白体液免疫反应。所有研究的患病犬均显示出IgG抗蛋白反应，而用作对照的健康犬中有2%（103只中的2只）呈阳性结果。通过ELISA检测发现呈阳性的所有血清在快速玻片凝集试验中也呈阳性。这些初步结果表明，使用流产布鲁氏菌细胞质蛋白的ELISA可用于犬布鲁氏菌病的特异性诊断。
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