Schmid E, Tapscott S, Bennett G S, Croop J, Fellini S A, Holtzer H, Franke W W
Differentiation. 1979;15(1):27-40. doi: 10.1111/j.1432-0436.1979.tb01031.x.
The location of constitutive proteins of different types of intermediate-sized (about 10 mm) filaments (cytokeratin, vimentin, desmin, brain filament protein) was examined in various tissues of 11--20 day chick embryos, using specific antibodies against the isolated proteins and immunofluorescence microscopy on frozen sections and on isolated serous membrane. The tissues studied which contained epithelia were small intestine, gizzard, esophagus, crop, liver, kidney, thymus, mesenteries, and epidermis. The results show that the different intermediate filament proteins, as seen in the same organ, are characteristic of specific lines of differentiation: Cytokeratin filaments are restricted to--and specific for--epithelial cells; vimentin filaments are seen--at this stage of embryogenesis--only in mesenchymal cells, including connective tissue, endothelial and blood cells, and chondrocytes; filaments containing protein(s) related to the subunit protein prepared from gizzard 10 nm filaments (i.e., desmin) are significant only in muscle cells; and intermediate filament protein of brain, most probably neurofilament protein, is present only in nerve cells. We conclude that for most tissues the expression of filaments of cytokeratin, vimentin, desmin, and neurofilament protein is mutually exclusive, and that these protein structurees provide useful markers for histochemical and cytochemical differentiation of cells of epithelial, mesenchymal, myogenic, and neurogenic differentiation.
利用针对分离蛋白的特异性抗体以及对冰冻切片和分离的浆膜进行免疫荧光显微镜检查，在11至20日龄鸡胚的各种组织中检测了不同类型中等大小（约10纳米）细丝（细胞角蛋白、波形蛋白、结蛋白、脑细丝蛋白）组成性蛋白的定位。所研究的含有上皮组织的组织有小肠、砂囊、食管、嗉囊、肝脏、肾脏、胸腺、肠系膜和表皮。结果表明，在同一器官中所见的不同中间丝蛋白是特定分化谱系的特征：细胞角蛋白丝局限于上皮细胞并对其具有特异性；波形蛋白丝在胚胎发育的这个阶段仅见于间充质细胞，包括结缔组织、内皮细胞和血细胞以及软骨细胞；含有与从砂囊10纳米细丝制备的亚基蛋白相关的蛋白（即结蛋白）的细丝仅在肌肉细胞中显著；而脑中间丝蛋白，很可能是神经丝蛋白，仅存在于神经细胞中。我们得出结论，对于大多数组织来说，细胞角蛋白、波形蛋白、结蛋白和神经丝蛋白细丝的表达是相互排斥的，并且这些蛋白质结构为上皮、间充质、肌源性和神经源性分化细胞的组织化学和细胞化学分化提供了有用的标记。
