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Crystallization and preliminary crystallographic analysis of the sarcosine oxidase from Bacillus sp. NS-129.

作者信息

Ichikawa T, Sasaki H, Koike H, Nishiyama M, Koyama Y, Tanokura M

机构信息

Biotechnology Research Center, University of Tokyo, Japan.

出版信息

J Struct Biol. 1997 Oct;120(1):109-11. doi: 10.1006/jsbi.1997.3906.

DOI:10.1006/jsbi.1997.3906
PMID:9356299
Abstract

The sarcosine oxidase from Bacillus sp. NS-129 has been crystallized by vapor diffusion using ammonium sulfate as precipitant. The tetragonal crystals have P4(1)2(1)2 (or P4(3)2(1)2) symmetry with a = b = 177.0 and c = 72.6 A. The crystals probably contain two sarcosine oxidase molecules per asymmetric unit and diffract to at least 2.7 A resolution.

摘要

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