模仿弧菌芳基酯酶的结晶及初步X射线晶体学分析

Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Vibrio mimicus.

作者信息

Musayev F N, Lee Y L, Shaw J F, Liaw Y C

机构信息

Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.

出版信息

Protein Sci. 1995 Sep;4(9):1931-3. doi: 10.1002/pro.5560040928.

DOI:10.1002/pro.5560040928

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2143213/

Abstract

Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53.46(1) A, c = 65.79(1) A, and beta = 106.54(1) degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.

摘要

在室温下，以硫酸铵作为沉淀剂，经过2个月已从模仿弧菌中获得了芳基酯酶（EC 3.1.1.2）的单晶。目前得到的晶体在2.2埃分辨率下仍能衍射，属于单斜空间群P2(1)。晶胞参数为a = 55.65(1) 埃，b = 53.46(1) 埃，c = 65.79(1) 埃，β = 106.54(1) 度。一个不对称单元中有两个分子量为22 kDa的分子，溶剂含量为43%。

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本文引用的文献

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