模仿弧菌芳基酯酶的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Vibrio mimicus.
作者信息
Musayev F N, Lee Y L, Shaw J F, Liaw Y C
机构信息
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
出版信息
Protein Sci. 1995 Sep;4(9):1931-3. doi: 10.1002/pro.5560040928.
Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53.46(1) A, c = 65.79(1) A, and beta = 106.54(1) degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.
在室温下，以硫酸铵作为沉淀剂，经过2个月已从模仿弧菌中获得了芳基酯酶（EC 3.1.1.2）的单晶。目前得到的晶体在2.2埃分辨率下仍能衍射，属于单斜空间群P2(1)。晶胞参数为a = 55.65(1) 埃，b = 53.46(1) 埃，c = 65.79(1) 埃，β = 106.54(1) 度。一个不对称单元中有两个分子量为22 kDa的分子，溶剂含量为43%。
Zotero 插件