Lindgren A C, Marcus C, Skwirut C, Elimam A, Hagenäs L, Schalling M, Anvret M, Lönnqvist F
Department of Woman and Child Health, Karolinska Hospital, Stockholm, Sweden.
Pediatr Res. 1997 Nov;42(5):593-6. doi: 10.1203/00006450-199711000-00007.
To study the potential role of the ob gene pathway in childhood obesity, we have investigated leptin mRNA levels in s.c. adipose tissue obtained from nonobese prepubertal children (n = 20), obese nonsyndromal children (n = 6), and children with Prader-Willi syndrome (n = 6) by in situ hybridization histochemistry. We have also investigated the fasting serum leptin levels in such children. Compared with nonobese children, leptin mRNA expression was higher both in children with Prader-Willi syndrome and in children with nonsyndromal obesity (p < 0.01). Furthermore, the serum leptin levels were also significantly higher in both children with Prader-Willi syndrome and nonsyndromal obesity compared with the nonobese children (p < 0.001). However, no significant differences in adipose tissue leptin mRNA or serum leptin levels were observed between children with Prader-Willi syndrome and nonsyndromal obese children. As expected both fasting serum leptin levels and leptin mRNA expression levels correlated to body mass index (rs = 0.80 and 0.73, respectively, p < 0.005). No difference in leptin expression between Prader-Willi syndrome and nonsyndromal childhood obesity could be revealed in the present study. However, differences in the hypothalamic response to leptin between the two forms of obesity cannot be excluded.
为研究ob基因通路在儿童肥胖中的潜在作用，我们通过原位杂交组织化学法，对取自非肥胖青春期前儿童（n = 20）、非综合征性肥胖儿童（n = 6）和普拉德-威利综合征儿童（n = 6）的皮下脂肪组织中的瘦素mRNA水平进行了研究。我们还对这些儿童的空腹血清瘦素水平进行了研究。与非肥胖儿童相比，普拉德-威利综合征儿童和非综合征性肥胖儿童的瘦素mRNA表达均较高（p < 0.01）。此外，与非肥胖儿童相比，普拉德-威利综合征儿童和非综合征性肥胖儿童的血清瘦素水平也显著更高（p < 0.001）。然而，未观察到普拉德-威利综合征儿童与非综合征性肥胖儿童在脂肪组织瘦素mRNA或血清瘦素水平上存在显著差异。正如预期的那样，空腹血清瘦素水平和瘦素mRNA表达水平均与体重指数相关（rs分别为0.80和0.73，p < 0.005）。在本研究中，未发现普拉德-威利综合征与非综合征性儿童肥胖之间的瘦素表达存在差异。然而，不能排除两种肥胖形式在下丘脑对瘦素反应方面的差异。
