Lipskaia L, Djiane A, Defer N, Hanoune J
Institut National de la Santé et de la Recherche Médicale U-99, Hôpital Henri Mondor, Créteil, France.
FEBS Lett. 1997 Oct 6;415(3):275-80. doi: 10.1016/s0014-5793(97)01001-6.
We have investigated the adenylyl cyclase (AC) activity and gene expression in retinoic acid (RA)-primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of neuroectodermal formation. Here we show that the P19 stem cells possess a basal Ca2+/CaM-stimulated AC activity, which increases about 10-fold after RA induction. The rise of AC activity is associated with a stage-specific up-regulation of AC2, AC5 and AC8 mRNAs and a down-regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the role and specific regulation of AC isoforms during neuronal differentiation.
