Normann P T, Norum K R
Scand J Gastroenterol. 1976;11(4):427-32.
Adult, male rats were given simultaneously 3H-mevalonate and 14C-cholesterol on autologous lipoproteins intravenously in barbital anaesthesia. The specific radioactivities of sterols in bile, liver and serum were determined. Newly synthesized hepatic cholesterol was a better substrate for cholic and chenodeoxycholic acid than was cholesterol reaching the liver via plasma lipoproteins. The cholesterol secreted into bile was derived from a pool of liver cholesterol which was in close contact with newly synthesized cholesterol. Specific radioactivity of chenodeoxycholate in bile was higher than that of cholate in bile, both when newly made radioactive cholesterol and when radioactive cholesterol on plasma lipoproteins was the precursor for the labelled bile acids.
成年雄性大鼠在巴比妥麻醉下，经静脉同时给予自体脂蛋白结合的3H-甲羟戊酸和14C-胆固醇。测定胆汁、肝脏和血清中固醇的比放射性。新合成的肝脏胆固醇比通过血浆脂蛋白进入肝脏的胆固醇更易成为胆酸和鹅去氧胆酸的底物。分泌到胆汁中的胆固醇来源于与新合成胆固醇密切接触的肝脏胆固醇池。无论是以新生成的放射性胆固醇还是以血浆脂蛋白上的放射性胆固醇作为标记胆汁酸的前体时，胆汁中鹅去氧胆酸盐的比放射性均高于胆酸盐的比放射性。
